List of Abstracts from Selected Papers

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The Effect of Allura red AC on the Motility and Regeneration Rate of Dugesia tigrina


Abstract: Red 40 dye has been banned in European countries due to rising health concerns. There is evidence that Red 40, also called Allura red AC, can cause hyperactivity in humans along with negatively affecting the colon and causing Early Onset Colorectal cancer in mice. This study aimed to investigate if doses of 0, 15, 30, and 60 microliters of Allura red AC can contribute to negative effects on the phototaxis rate and the rate of regeneration of Dugesia tigrina. It is hypothesized that if Allura red AC is fed to Dugesia tigrina then, it will increase motility, and slow the rate of regeneration. This study was performed using four groups of Dugesia tigrina with 0 µM, 0.125µM, 0.25µM, and 0.5µM of red 40 and measuring the length of the planaria, and the rate of movement of planaria over the course of 31 days. The results of this study show that an increase in Allura red AC exposure causes a decrease in the regeneration rate, an increase in phototaxis, and an increase in mortality. Future research suggests a conversion in dosage and/or form of dye (powder version). Also, recommendations for future research include using a different type of organism such as Drosophila melanogaster. 


References

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CDC. “What Are the Risk Factors for Colorectal Cancer?” Centers for Disease Control and Prevention, 2019, www.cdc.gov/cancer/colorectal/basic_info/risk_factors.htm. Accessed 4 Mar. 2024. 

Center for Food Safety and Applied Nutrition. “Color Additives History.” U.S. Food and Drug Administration, 3 Nov. 2017, www.fda.gov/industry/color-additives/color-additives-history. Accessed 4 Mar. 2024.

Centers for Disease Control and Prevention. “What Is ADHD?” Centers for Disease Control and Prevention, 27 Sept. 2023, www.cdc.gov/ncbddd/adhd/facts.html. Accessed 4 Mar. 2024. Cleveland Clinic. “Is Red Dye 40 Safe?” Cleveland Clinic, 8 Mar. 2023, health.clevelandclinic.org/red-dye-40. Accessed 4 Mar. 2024. 

Issigonis, Melanie. “Could We Use Planarians to Help Us Understand Human Regeneration?” Morgridge Institute for Research, 24 Dec. 2017, morgridge.org/blue-sky/could-we-use-planarians-to-help-us-understand-human-regeneration/#:~:text=But%2C%20unlike%20planarians%2C%20humans. Accessed 4 Mar. 2024.

Kanarek, Robin B. “Artificial Food Dyes and Attention Deficit Hyperactivity Disorder.” Nutrition Reviews, vol. 69, no. 7, 30 June 2011, pp. 385–391, https://doi.org/10.1111/j.1753-4887.2011.00385.x. 

Newmark, Phillip. “Flatworms at Forefront of Regeneration Research.” Www.nsf.gov, 7 July 2006, 

Paskin, Taylor R., et al. “Planarian Phototactic Assay Reveals Differential Behavioral Responses Based on Wavelength.” PLoS ONE, vol. 9, no. 12, 10 Dec. 2014, p. e114708, https://doi.org/10.1371/journal.pone.0114708. 

“Potential Neurobehavioral Effects of Synthetic Food Dyes in Children.” Ca.gov, 2021, oehha.ca.gov/media/downloads/risk-assessment/report/healthefftsassess041621.p. Accessed 4 Mar. 2024. 

Sagon, Candy. “8 Foods We Eat That Other Countries Ban.” Blogs, 25 June 2013, blog.aarp.org/healthy-living/8-foods-we-eat-that-other-countries-ban. Accessed 4 Mar. 2024.

Sarnat, Harvey B., and Martin G. Netsky. “The Brain of the Planarian as the Ancestor of the Human Brain.” Canadian Journal of Neurological Sciences / Journal Canadien Des Sciences Neurologiques, vol. 12, no. 4, Nov. 1985, pp. 296–302, https://doi.org/10.1017/s031716710003537x. 

Vorhees, C V, et al. “Developmental Toxicity and Psychotoxicity of FD and c Red Dye No. 40 (Allura Red AC) in Rats.” Toxicology, vol. 28, no. 3, 1983, pp. 207–17, www.ncbi.nlm.nih.gov/pubmed/6636206/, 

Walsh, Caroline J., et al. “Obstetric Complications in Mothers with ADHD.” Frontiers in Reproductive Health, vol. 4, 7 Nov. 2022, https://doi.org/10.3389/frph.2022.1040824. Accessed 4 Mar. 2024. 

“What Is Red Dye 40? ADHD and Brain Health | Amen Clinics.” Brain Health Guide to Red Dye #40, 24 Aug. 2023, www.amenclinics.com/blog/brain-health-guide-red-dye-40/#:~:text=The%20use%20of% 20Red%20Dye. Accessed 4 Mar. 2024. 

Wirth, Jennifer. “ADHD Statistics.” Forbes Health, 6 June 2023, www.forbes.com/health/mind/adhd-statistics/#:~:text=An%20estimated%208.7%20milli on%20adults. Accessed 4 Mar. 2024. 

Zhang, Qi, et al. “The Synthetic Food Dye, Red 40, Causes DNA Damage, Causes Colonic Inflammation, and Impacts the Microbiome in Mice.” Toxicology Reports, vol. 11, 1 Dec. 2023, pp. 221–232, www.sciencedirect.com/science/article/pii/S2214750023000926, https://doi.org/10.1016/j.toxrep.2023.08.006. Accessed 19 Sept. 2023.

Water Retention of Small-Scale Green Roofs with Edible Vegetation


Abstract: 

Green roofs (GRs) are typically used to retain stormwater and are increasingly being used to produce food by growing edible vegetation, such as Mad Hatter Peppers (Capsicum baccatum). However, there have been conflicting studies on whether GRs can feasibly produce Capsicum baccatum in GRs compared to in-ground production. To test this, water retention was compared among small-scale models of three different vegetation types: two Sedum setups, two Capsicum baccatum setups, and one bare setup. The models used water storage compartments and moisture retention fabric to increase water retention and to reduce the need for irrigation. There was not a statistically significant difference in water retention between the different vegetation types, and the Capsicum baccatum wilted by the end of the study, so it did not produce food. These results indicate that Sedum should be used in future GRs because they can provide many benefits other than water retention, whereas Capsicum baccatum may not be healthy enough to provide other benefits.


References

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Eksi, M., Rowe, D. B., Fernández-Cañero, R., & Cregg, B. M. (2015). Effect of substrate compost percentage on green roof vegetable production. Urban Forestry & Urban Greening, 14(2), 315–322. https://doi.org/10.1016/j.ufug.2015.03.006 

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